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NAD+

NAD+ is a coenzyme central to cellular energy production, DNA repair, and anti-aging sirtuin activation. Injectable NAD+ restores declining levels directly.

Overview

NAD+ participates in over 500 enzymatic reactions and is essential for mitochondrial electron transport chain function. Age-related NAD+ decline of 10-50% across tissues has been linked to metabolic dysfunction, neurodegeneration, and accelerated aging.

Mechanism of Action

NAD+ restores cellular energy production through mitochondrial electron transport, activates sirtuins (SIRT1-7) for DNA repair and longevity signaling, and supports PARP-mediated genome maintenance.

  • Mitochondrial Electron Transport: Primary electron carrier in respiratory chain, driving ATP synthesis
  • Sirtuin Activation (SIRT1-7): Essential co-substrate for all seven mammalian sirtuins regulating gene expression and DNA repair
  • PARP-Mediated DNA Repair: Consumed by PARP enzymes to catalyze DNA break repair and genome maintenance
  • CD38 / NAD+ Homeostasis: Counteracts age-related NAD+ depletion driven by increased CD38 activity
  • Circadian Rhythm Regulation: NAD+ oscillations drive SIRT1-mediated regulation of CLOCK/BMAL1

Reconstitution Calculator

NAD+

Nicotinamide adenine dinucleotide (NAD+) is a vital coenzyme present in every li

Draw Volume
0.600mL
Syringe Units
60units
Concentration
166,667mcg/mL
Doses / Vial
5doses
Vial Total
500mg
Waste / Vial
0mcg
Syringe Cap.
100units · 1mL
Recommended Schedule
M
T
W
T
F
S
S
Frequencydaily
TimingMorning
Cycle8-16 weeks
NoteRamp-up: 50mg daily week 1, 75mg daily week 2, then 100mg daily from week 3 onward
How to reconstitute
Gather & prepare
1/6Gather & prepare

Set up a clean workspace with all supplies ready.

1.Wash hands thoroughly, put on disposable gloves
2.Your 500mg peptide vial (lyophilized powder)
3.Bacteriostatic water (you'll need 3mL)
4.A 3–5mL syringe with 21–25 gauge needle for reconstitution
5.Alcohol swabs (70% isopropyl)
Use bacteriostatic water (0.9% benzyl alcohol) for multi-dose vials. Sterile water is only safe for single-use.
Supply Planner

7x / week for weeks

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60%
6vials
28 doses5 days/vial2 leftover
Cost Breakdown
Vial price
$0.00per dose
$0.00 /week$0 /month
Store 2-8°C30 day shelf lifeSwirl gentlyFor research purposes only

This calculator is provided for informational and educational purposes only. It is not intended as medical advice, diagnosis, or treatment guidance. Always consult a qualified healthcare professional before preparing or administering any substance. PepGuide assumes no liability for decisions made based on these calculations.

Research

Anti-Aging & Longevity

NAD+ decline of 10-50% during aging. Supplementation restores sirtuin activity and DNA repair.

  • Age-related decline across tissues
  • Sirtuin activation extends lifespan
  • PARP DNA repair restored

Neuroprotection

Reduces neuroinflammation and supports neurotransmitter synthesis.

  • Intranasal NAD+ protected hippocampal neurons in TBI
  • NR-SAFE trial positive in Parkinsons

Metabolic Health

Central to glucose and lipid metabolism via mitochondrial function.

  • NMN RCT: dose-dependent performance improvements
  • Improved insulin sensitivity

Cognitive Enhancement

Supports neuronal energy, synaptic plasticity, and neurotransmitter optimization.

  • Enhanced mitochondrial function in neurons
  • SIRT1 promotes neuronal survival

Safety Profile

Start with lower doses (50mg) to evaluate tolerance. IV requires medical supervision. Avoid alcohol during therapy. Refrigerate reconstituted solution and use within 14 days.

Pharmacokinetic Profile

NAD+ — Pharmacokinetic Curve

Subcutaneous injection
0%25%50%75%100%0m45m1.5h2.3h3h3.8hTimeConcentration (% peak)T_max 25mT_1/2 45m
Half-life: 45mT_max: 30mDuration shown: 3.8h

Quick Start

Typical Dose
100mg daily (after 2-week ramp-up)
Frequency
Once daily
Route
Subcutaneous injection
Timing
Morning preferred
Cycle Length
8-16 weeks
Break Between Cycles
4-8 weeks
Storage
Refrigerate 2-8°C, use within 14 days
Injection Sites
Abdomen · Thigh · Upper arm

Molecular Structure

2D Structure
NAD+ molecular structure
Molecular Properties
Formula
C21H27N7O14P2
Weight
663.43 Da
Length
2 amino acids
CAS
53-84-9
PubChem CID
5892

Research Indications

Primary

Emerging

Directly restores declining NAD+ levels

Emerging

Supports neuronal energy and neuroprotection

Emerging

Central role in mitochondrial function

Secondary

Emerging

Restores mitochondrial ATP production

Emerging

Early clinical trials in Parkinsons

Emerging

Enhanced mitochondrial recovery

Research Protocols

Subcutaneous Injection

100mg daily subcutaneous injection. Ramp up: 50mg week 1, 75mg week 2, then 100mg daily.

GoalDoseFrequency
100mgDaily

Intramuscular Injection

250-500mg IM injection 1-2x weekly. 80-90% bioavailability.

GoalDoseFrequency
250-500mg1-2x weekly

Intravenous Injection

500-1000mg IV infusion 1-2x weekly. Clinical setting required. 100% bioavailability.

GoalDoseFrequency
500-1000mg1-2x weekly

Intranasal Injection

25-50mg intranasal 1-2x daily. Bypasses blood-brain barrier.

GoalDoseFrequency
25-50mg1-2x daily

Interactions

Peptide Interactions

✦
BPC-157synergistic

Enhances cellular repair and energy production

✦
TB-500synergistic

Complementary tissue regeneration and mitochondrial support

✦
Ipamorelinsynergistic

GH pathways benefit from improved cellular energy

✦
L-Carnitinesynergistic

Both target mitochondria via complementary mechanisms

What to Expect

What to Expect

Week 1-2

Initial energy improvements, enhanced mental clarity, possible mild flushing

Week 3-4

Enhanced physical performance and faster recovery

Week 5-8

Sustained energy, improved sleep quality

Week 9-12+

Optimized metabolic function, cumulative cognitive benefits

Safety Profile

Common Side Effects

  • Injection site redness or irritation
  • Mild nausea
  • Warmth or flushing sensation
  • Mild headache

Contraindications

  • Active cancer
  • Pregnancy or breastfeeding
  • Known hypersensitivity to NAD+ or nicotinamide

Drug Interactions

  • Alcohol substantially depletes NAD+ levels — avoid
  • May interact with chemotherapy agents targeting NAD+ metabolism

Uncommon

  • Chest tightness during IV infusion
  • Muscle cramping
  • Lightheadedness

Quality Indicators

What to look for

  • White to off-white lyophilized powder
  • Dissolves to clear colorless solution
  • Third-party COA with HPLC purity ≥98%
  • Shipped with cold packs

Caution

  • Yellow tint may indicate degradation
  • Use within 14 days of reconstitution

Red flags

  • Cloudy or discolored solution
  • Visible particles
  • Stored at room temperature
  • No COA available

Frequently Asked Questions

References (5)

  1. [1]
    Won SJ et al. Intranasal NAD+ for traumatic brain injury Journal of Neurotrauma (2012)

    → Protected hippocampal neurons

  2. [2]
    Martens CR et al. Chronic NR supplementation elevates NAD+ Nature Scientific Reports (2019)

    → Safe NAD+ elevation in adults

  3. [3]
    Massudi H et al. Age-associated NAD+ metabolism changes PLoS ONE (2012)

    → NAD+ decreases 10-50% with age

  4. [4]
    Trammell SA et al. NR augments human blood NAD+ Nature Communications (2016)

    → Dose-dependent NAD+ elevation

  5. [5]
    Yi L et al. NMN in middle-aged adults RCT GeroScience (2022)

    → Dose-dependent performance gains

Sources: PubMed, pep-pedia.org, peptidedosages.com

N-Acetyl Semax

N-Acetyl Semax is a synthetic peptide derivative of ACTH (adrenocorticotropic hormone) that acts as a neuropeptide with cognitive-enhancing and neuroprotective

NADH

NADH (nicotinamide adenine dinucleotide hydride) is the reduced form of NAD+, a critical coenzyme involved in cellular energy production, redox reactions, and m

On this page

Overview
Reconstitution Calculator
Mechanism of Action
Research
Anti-Aging & Longevity
Neuroprotection
Metabolic Health
Cognitive Enhancement
Safety Profile
Pharmacokinetic Profile
Quick Start
Molecular Structure
Research Indications
Research Protocols
Interactions
What to Expect
Safety Profile
Quality Indicators
FAQ
References
Related Peptides