XANTHOHUMOL

Xanthohumol is a prenylated chalcone flavonoid primarily found in hops (Humulus lupulus), possessing broad biological activities including antioxidant, anti-inflammatory, and anticancer properties. It modulates cellular defense mechanisms and induces apoptosis in cancer cells.

Xanthohumol is a prenylated flavonoid compound derived from hops (Humulus lupulus) with potent antioxidant, anti-inflammatory, and neuroprotective properties. It works primarily by regulating the Nrf2/NF-κB/mTOR/AKT pathways, reducing oxidative stress, inhibiting inflammation, and potentially protecting against neurodegenerative diseases and metabolic disorders. Research suggests benefits for cognitive function, metabolic health, and disease prevention, though human clinical data remains limited.

Research

Reported Effects

Cognitive Enhancement:: Animal studies show consistent improvements in cognitive flexibility and protection against age-related brain damage, though human data is limited. Microbiome Dependent:: Effectiveness for metabolic benefits appears dependent on intact gut microbiota, suggesting variable individual responses. Tau Aggregation:: In vitro studies show promise for Alzheimer's prevention through direct tau protein interaction, but clinical translation uncertain. Safety Profile:: Extensive animal studies show good safety at high doses with no organ toxicity, supported by Phase I human trial data

  • Animal studies show consistent improvements in cognitive flexibility and protection against age-related brain damage, though human data is limited
  • Effectiveness for metabolic benefits appears dependent on intact gut microbiota, suggesting variable individual responses
  • In vitro studies show promise for Alzheimer's prevention through direct tau protein interaction, but clinical translation uncertain
  • Extensive animal studies show good safety at high doses with no organ toxicity, supported by Phase I human trial data

Safety Profile

Safety Profile: Xanthohumol

Common Side Effects

  • Limited human clinical trial data; most safety information from in vitro and animal studies
  • Mild gastrointestinal discomfort including nausea, bloating, and diarrhea
  • Headache reported in some study participants
  • Mild estrogenic or anti-estrogenic effects (xanthohumol is a prenylated flavonoid from hops with complex hormonal activity)
  • Dietary exposure through beer is minimal (0.1–0.5 mg/L) and generally poses no safety concerns

Serious Adverse Effects

  • Estrogenic activity: Xanthohumol's metabolite isoxanthohumol can be converted to 8-prenylnaringenin (the most potent known phytoestrogen) by gut bacteria; this raises concerns for hormone-sensitive conditions
  • Hepatotoxicity: High doses showed liver stress markers in some animal studies; clinical relevance in humans uncertain
  • Limited pharmacokinetic data in humans; bioavailability is low and variable
  • Potential immunomodulatory effects that are not fully characterized in vivo
  • Allergic reactions possible in individuals sensitive to hops (Humulus lupulus) or related plants

Contraindications

  • Known hypersensitivity to hops (Humulus lupulus) or Cannabaceae family plants
  • Hormone-sensitive conditions (breast cancer, endometrial cancer, endometriosis) due to phytoestrogenic metabolites
  • Active liver disease (limited safety data; hepatotoxicity concern from animal studies)
  • Pregnancy and lactation (estrogenic activity and insufficient safety data)
  • Concurrent use of hormone replacement therapy without medical supervision

Drug Interactions

  • Estrogen and hormonal therapies (HRT, oral contraceptives): Potential estrogenic interaction via 8-prenylnaringenin metabolite
  • CYP1A2 and CYP3A4 substrates: Xanthohumol may modulate these enzymes in vitro; clinical significance not established but caution warranted
  • Anticoagulants/Antiplatelets: Some prenylated flavonoids have antiplatelet properties; monitor for bleeding
  • Tamoxifen and aromatase inhibitors: Estrogenic metabolites may counteract anti-estrogen cancer therapies
  • Sedatives and anxiolytics: Hops-derived compounds may have mild sedative effects; additive CNS depression possible

Population-Specific Considerations

  • Menopausal women: Potential benefit for menopausal symptoms via phytoestrogenic activity, but use cautiously in those with hormone-sensitive cancer history
  • Elderly: Limited safety data; start with low doses
  • Pediatric: No safety data; not recommended
  • Beer consumers: Dietary exposure from beer is far below supplemental doses; no additional safety concerns
  • Cancer patients: Complex: anticancer properties demonstrated in vitro, but estrogenic metabolite is a concern for hormone-sensitive cancers; consult oncologist

Pharmacokinetic Profile

Molecular Structure

2D Structure
XANTHOHUMOL molecular structure
Molecular Properties
Formula
C21H22O5
Weight
354.4 Da
PubChem CID
639665
Exact Mass
354.1467 Da
LogP
5.1
TPSA
87 Ų
H-Bond Donors
3
H-Bond Acceptors
5
Rotatable Bonds
6
Complexity
514
Identifiers (SMILES, InChI)
InChI
InChI=1S/C21H22O5/c1-13(2)4-10-16-18(24)12-19(26-3)20(21(16)25)17(23)11-7-14-5-8-15(22)9-6-14/h4-9,11-12,22,24-25H,10H2,1-3H3/b11-7+
InChIKeyORXQGKIUCDPEAJ-YRNVUSSQSA-N

Safety Profile

Common Side Effects

  • Minimal Reported:: Animal safety studies at very high doses (1000 mg/kg) showed no significant adverse effects or organ damage
  • Individual Variation:: Effects may vary based on gut microbiome composition and metabolic status
  • Drug Interactions:: Potential effects on CYP3A11 enzyme expression suggest possible interactions with medications metabolized by this pathway
  • Long-term Safety:: Good safety profile in animal studies, but limited long-term human data available from clinical trials

References (9)

  1. [1]
    Antioxidant Potential of Xanthohumol in Disease Prevention: Evidence from Human and Animal Studies

    Comprehensive review showing xanthohumol's strong antioxidant and anti-inflammatory effects through regulation of key cellular pathways, with potential for preventing cancer and neurodegenerative diseases, though some risks exist in certain disease states like advanced prostate cancer.

  2. [2]
    Xanthohumol improved cognitive flexibility in young mice

    Study demonstrated that xanthohumol supplementation improved cognitive flexibility and reversed age-related changes in protein palmitoylation status in brain tissue, suggesting potential neuroprotective benefits.

  3. [3]
    Protective effect of xanthohumol against age-related brain damage

    Research found xanthohumol protected against age-related brain damage through antioxidant mechanisms, reducing oxidative stress markers and improving mitochondrial function in aging brain tissue.

  4. [4]
    Xanthohumol protects neuron from cerebral ischemia injury in experimental stroke

    Animal studies showed xanthohumol provided neuroprotection in stroke models through antioxidant effects, reducing brain damage and improving outcomes following ischemic injury.

  5. [5]
    Xanthohumol inhibits tau protein aggregation and protects cells against tau aggregates

    Study found xanthohumol directly inhibits tau protein aggregation and disaggregates tau fibrils, protecting cells from tau-induced apoptosis with potential implications for Alzheimer's disease prevention.

  6. [6]
    Xanthohumol Requires the Intestinal Microbiota to Improve Glucose Metabolism in Diet-Induced Obese Mice

    Research demonstrated that xanthohumol's beneficial effects on glucose metabolism and obesity are mediated through the gut microbiome, with effects being lost in microbiota-depleted animals.

  7. [7]
    Xanthohumol microbiome and signature in healthy adults (the XMaS trial): a phase I triple-masked, placebo-controlled clinical trial

    Phase I clinical trial examining the safety and tolerability of xanthohumol supplementation in healthy adults, with focus on microbiome effects and metabolic markers.

  8. [8]
    Xanthohumol feeding does not impair organ function and homoeostasis in mice

    Safety study found that high-dose xanthohumol supplementation (1000 mg/kg body weight) for 3 weeks showed no signs of toxicity or organ damage in mice, supporting its safety profile.

  9. [9]
    Xanthohumol, a hop-derived prenylated flavonoid, promotes macrophage reverse cholesterol transport

    Study showed xanthohumol increased reverse cholesterol transport in a hamster model, potentially contributing to its anti-atherosclerotic effects and cardiovascular benefits.

Updated 2026-03-08Sources: peptidebay, pubchem

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