Apraglutide

A next-generation, long-acting glucagon-like peptide-2 (GLP-2) analog engineered for once-weekly dosing in short bowel syndrome.

Overview

Apraglutide is designed to deliver sustained GLP-2 receptor stimulation with far less frequent dosing than teduglutide. Four amino-acid substitutions relative to native GLP-2 provide DPP-4 resistance, and high plasma-protein binding lowers clearance, extending the elimination half-life to roughly 72 hours—enough for a once-weekly regimen.

A placebo-controlled phase 2 crossover trial in adults with short bowel syndrome and intestinal failure found that once-weekly apraglutide significantly increased urine output, a marker of improved intestinal fluid absorption, and was well tolerated at both 5 mg and 10 mg doses. It subsequently advanced into the phase 3 STARS program in SBS-IF, where it became the first once-weekly GLP-2 analog to show efficacy in reducing parenteral-support needs.

As an intestinotrophic agent, its safety considerations parallel the GLP-2 class, including monitoring for colorectal polyps and gastrointestinal or fluid-balance effects.

Mechanism of Action

Like teduglutide, apraglutide acts through GLP-2 receptors expressed on subepithelial myofibroblasts and enteroendocrine cells rather than directly on enterocytes, promoting epithelial proliferation via mediators such as IGF-1. Its engineered pharmacokinetics aim to provide more constant receptor exposure across a weekly interval, which may improve absorptive outcomes and adherence compared with daily GLP-2 therapy.

Reconstitution Calculator

Apraglutide

Apraglutide is a synthetic GLP-2 analog with four amino-acid substitutions and h

Draw Volume
0.500mL
Syringe Units
50units
Concentration
10,000mcg/mL
Doses / Vial
1doses
Vial Total
5mg
Waste / Vial
0mcg
Syringe Cap.
100units · 1mL
Recommended Schedule
M
T
W
T
F
S
S
Frequencyweekly
Cycleongoing
How to reconstitute
Gather & prepare
1/6Gather & prepare

Set up a clean workspace with all supplies ready.

1.Wash hands thoroughly, put on disposable gloves
2.Your 5mg peptide vial (lyophilized powder)
3.Bacteriostatic water (you'll need 0.5mL)
4.A 3–5mL syringe with 21–25 gauge needle for reconstitution
5.Alcohol swabs (70% isopropyl)
Use bacteriostatic water (0.9% benzyl alcohol) for multi-dose vials. Sterile water is only safe for single-use.
Supply Planner

1x / week for weeks

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4vials
4 doses7 days/vial
Cost Breakdown
Vial price
$0.00per dose
$0.00 /week$0 /month
Store 2-8°C30 day shelf lifeSwirl gentlyFor research purposes only

This calculator is provided for informational and educational purposes only. It is not intended as medical advice, diagnosis, or treatment guidance. Always consult a qualified healthcare professional before preparing or administering any substance. PepGuide assumes no liability for decisions made based on these calculations.

References (1)

  1. [1]
    Eliasson J, Hvistendahl MK, Freund N, et al. Apraglutide, a novel glucagon-like peptide-2 analog, improves fluid absorption in patients with short bowel syndrome intestinal failure: Findings from a placebo-controlled, randomized phase 2 trial JPEN Journal of Parenteral and Enteral Nutrition (2022)

    Once-weekly apraglutide significantly increased intestinal fluid absorption (urine output) in short bowel syndrome with intestinal failure and was well tolerated.

Updated 2026-07-07Reviewed by ai-enrich-2026-07-contentSources: https://pubmed.ncbi.nlm.nih.gov/34287970/

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